The breeding world can be a challenging and stressful world to enter. Our team of doctors and skilled reproduction assistants are here to help you through the entire process. Feel free to contact our office with any questions at(626) 796-8387.
Pre - Breeding Exams
We require a pre-breeding office visit prior to breeding. All bitches need to be examined by a doctor so that you can discuss your breeding plan, i.e. semen type, history, what type of breeding etc.. This is the best time to ask any questions that you may have. The doctor will also discuss any testing that may need to be performed i.e. brucellosis, OFA x-rays, vaginal cultures or thyroid testing. We recommend that you schedule your pre-breeding exam for a time just prior to when you expect your bitch to come into season.
Please feel free to contact one of our reproductive assistants at any time. They are very knowledgeable and can answer most of your questions regarding your breeding at no charge. If you would prefer to have a telephone consultation with one of our doctors, we can set up a 15 minute telephone consultation for a $35 fee.
Determining Breeding Dates
Breeding dates are determined with various factors in mind. Type of semen, number of inseminations, type of insemination, and bitches history can all influence breeding dates.
We recommend that progesterone testing be started between 5-7 days into the heat cycle.
The progesterone tests that we run will help us to determine ovulation. We also obtain a vaginal cytology sample or “smear” with a sterile swab from the vaginal lining when we draw the blood for the progesterone test. The progesterone level from the blood gives us an indication of when the bitch ovulates. There is no single number for determining the time of ovulation; we are looking for a number between 4ng/ml to 8ng/ml as the start of ovulation. Most typical bitches ovulate around 5ng/ml. The results are evaluated by the doctor keeping in mind the speed of rise, the type of breeding and type of semen. Many times the doctors will recommend rechecking the progesterone level after the level has reached a level of 5-8 in order to confirm rise and the rate of the progesterone rise especially with frozen semen breedings.
For frozen semen breedings, we also use LH (lutenizing hormone) testing. The LH peak occurs 48 hours prior to ovulation and the hormone is only detectable for 24 hours. In order to perform the test, we draw blood every day at the same time. We separate the serum and freeze it daily. We continue to test the progesterone levels every 2-3 days and when the doctors feel that the bitch has ovulated, we will test the LH on the frozen serum that was draw 2 days prior to the suspected ovulation. If the LH test is (+) then we have confirmed that the ovulation occurred the day they suspected. If the LH test is (-) then they will determine whether the test should be repeated on the day before or after the suspected LH peak. There are rare occasions when the LH peak is not detected
After ovulation, the eggs need time to mature or “ripen” so that they can be fertilized. The eggs are typically ready 48 hours after ovulation and can be fertilized for several days. This is where the type of sperm can change the breeding dates. We can expect fresh collected semen to last for 24-48 hours or longer, fresh chilled around 24 hours after insemination and frozen around 12 hours. Of course, the quality of the semen is a big determining factor in this equation. Typically, we start breeding a day later for frozen semen and prefer two, sometimes three breeding for maximum conception rates.
Progesterone testing is provided on a walk in basis by our in house laboratory.
If you arrived by 12pm for your test, the doctor’s recommendation will be available after 4pm the same day. You may be asked to recheck another test within 1-3 days or a recommendation for breeding dates will be relayed to you.
If you arrive after 12pm, your results will be ready by 11am the following business day.
Saturday progesterone tests available before 12pm for same day results, tests requested after 12pm results will be returned Monday.
If you need results earlier in the day or you cannot come in until after 12pm (before 3pm) and still need same day results, we can run a STAT (faster) test for an additional $30 and your results will be available in 2-3 hours. STAT's after 3pm are at office discretion due to maintenance requirements of the lab equipment.
Semen Evaluation (SpermVision) - We are one of very few hospitals that have the Sperm Vision technology. This technologically advanced system gives a more accurate analysis of semen, analyzing motility, abnormalities and concentration. This process allows owners to better understand the viability of their pet’s semen collection.
Chilled Semen Shipping - This process enables the stud dog owner to ship the sperm anywhere within the United States or Canada, typically overnight.
Semen Freezing and Storage - We have the resources to collect and freeze canine semen, and provide reliable long-term storage.
Frozen Semen Shipping (International and Domestic) - This process enables an owner to send canine semen anywhere in the world, unless international restrictions apply. Call for information prior to semen collection regarding specific country regulations.
Board-for-Breeding is an opportunity for busy owners to board the bitch from the beginning of her heat cycle until after she is bred. We perform all necessary tests per doctor’s directions, and notify the owner of all results.
Surgical Inseminations – This procedure is recommended for females 6 years of age and over, and for females who have failed to conceive previously. It may also be beneficial when using frozen semen to inseminate.
Trans-Cervical Inseminations – This is the most commonly used insemination procedure offered at our hospital. It requires no anesthesia, and places semen through the cervix into the uterus using an endoscopic camera.
Vaginal Inseminations – This procedure places semen into the vaginal vault. It is usually recommended when using high quality fresh or chilled semen.
Ultrasound for Pregnancy - Pregnancies can be safely detected on ultrasound. This is usually best done between days 28 to 35 after the first breeding. Ultrasound can also be used to estimate puppy count and monitor heart beats.
X-Ray for Puppies - During the last week of pregnancy, x-rays are often used for a more accurate count of puppies. It can be used to determine the safest birth route. X-ray can also be used to determine if there are any more puppies post-whelping.
When you know that your bitch will need a c-section because of breed, past history, risk factors or size of litter, we can schedule your surgery in advance. The c-section date(s) will be determined by the doctor according to the breeding dates, and patients’ history in conjunction with progesterone levels.
If you are planning a surgery and have been asked to come in for progesterone testing, you need to have the blood drawn by 9:30 am and have the test run STAT for faster results ($30 additional fee). The decision as to whether the c-section will proceed that day or be postponed, needs to be determined by 12:00pm in order to prevent after hours charges from being incurred. Our regular surgery hours end at 3pm.
Sometimes, a c-section is required unexpectedly, when this occurs, we do our best to accommodate our clients and patients according to the level of urgency. If the decision to do a c-section is made early in the day, and you arrive before 12pm, we can usually get the surgery done by 3pm and avoid after hours fees. ALL C-SECTIONS PERFORMED AFTER 3PM, OR DURING NON-BUSINESS HOURS WILL BE CHARGED AFTER HOURS RATES.
On many occasions, we can do c-sections when our office is closed, on Sundays or on holidays when arranged in advance.
After hours’ fees are charged in order for us to provide an adequate staff for the safety of your bitch and the puppies.